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Image Search Results
Journal: Cell reports
Article Title: Phenotypic Screen with TSC-Deficient Neurons Reveals Heat-Shock Machinery as a Druggable Pathway for mTORC1 and Reduced Cilia
doi: 10.1016/j.celrep.2020.107780
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, Plasmid Preparation, cDNA Synthesis, SYBR Green Assay, RNA Sequencing, Expressing, Luciferase, Software, Cell Analysis
Journal: Molecular Oncology
Article Title: The antiviral drug telaprevir induces cell death by reducing FOXA1 expression in estrogen receptor α ( ERα )‐positive breast cancer cells
doi: 10.1002/1878-0261.13303
Figure Lengend Snippet: Analysis of telaprevir‐induced apoptosis. (A) Western blotting and (A’) relative densitometric analyses of cleaved PARP and tubulin (Tub) in MCF‐7 cells treated with different doses of telaprevir (Tel 10–40 μ m ) for 48 h and staurosporine (STS 100 n m ) for 24 h. Data are the mean ± standard deviation with P ‐value < 0.0001. n = 4 for all samples but for 40 μ m where n = 3. **** indicates significant differences with respect to the control sample (0) calculated with one‐way ANOVA followed by the Tukey post‐test. Western blotting analyses of telaprevir (Tel) effect on cleaved PARP, FOXA1 and tubulin (Tub) levels in the presence of FOXA1 siRNA (B) and NVP (C). Cells were transfected with FOXA1 siRNA and treated with 1 μ m NVP before 48 h of 20 μ m Tel treatment. (D) Western blotting analyses of cleaved PARP, FOXA1 and vinculin expression in MCF‐7 cells pretreated with different doses of IGF (10–250 ng·mL −1 ) for 1 h before 48 h of 20 μ m Tel treatment. Densitometric analyses of panel (B) (C) and (D) are shown in Fig. C–H. n = 4 for (B) (C) and (D), n = 3 for FOXA1 levels in panel D. (E–H) Western blotting analyses of cleaved PARP and tubulin in HeLa, SKOV3, MCF‐7, and SKBR3 cells treated with telaprevir (Tel 20 μ m ) for 48 h. Densitometric analysis are shown in Fig. B. n = 3 for all the cell lines and n = 4 for SKOV3 cells.
Article Snippet: Antibodies against FOXA1/HNF3α (53528S, rabbit), phospho‐AKT Ser473 (4058S, rabbit),
Techniques: Western Blot, Standard Deviation, Control, Transfection, Expressing